A research team has created a fluorescent marker molecule that does not degrade under a STED microscope: the photobleaching problem has been solved. Images of living cells can now be captured over ...

A decade ago, the Nobel Prize in Chemistry was awarded to a trio of researchers for the development of super-resolved fluorescence microscopy. The announcement at the time stated that the researchers’ ...

Stimulated emission depletion (STED) microscopy has shown that diffraction-unlimited spatial resolution is viable with conventional lenses and visible light. Stefan W Hell, Lars Kastrup and Katrin I ...

Raman microscopy combines optical microscopy with the ability to determine the chemical makeup of surfaces. Laser light is delivered to the sample surface via the same objective as the optical light ...

It’s relatively easy to understand how optical microscopes work at low magnifications: one lens magnifies an image, the next magnifies the already-magnified image, and so on until it reaches the eye ...

While confocal microscopy uses a pinhole to reject out-of-focus light to generate the optical section, a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence ...

Visualizing tissues and neurons inside an animal's skull has long been a challenge for biophotonics techniques, thanks to the skull's thickness and the complex structures inside. A team at the ...

Swiss researchers have developed a very-high-resolution x-ray microscope. Their approach combines two well-known microscopy techniques, coherent diffractive imaging (CDI) and scanning transmission ...

Fluorescence light microscopy techniques offer several advantages when imaging biological samples, including high image contrast, good labeling specificity, multicolor, and three dimensional (3D) ...